| Primer . | Sequence (5ʹ–3ʹ) . | Purpose . |
|---|---|---|
| pmela-Cas9-F | CTTGACCTCTCCTTCCACATTTGAC | Positive gene knockout fish screening |
| pmela-Cas9-R | TGCTGCTAAGCTCTTATGGGG | Positive gene knockout fish screening |
| pmelb-Cas9-F | CCAAAAACCGCTTCACTCGTT | Positive gene knockout fish screening |
| pmelb-Cas9-R | TCTTCCTGTGGGGACTGACC | Positive gene knockout fish screening |
| pmela-page-F | AGCAAAAGCGGGCTTCACTA | F1 and F2 mutant fish screening |
| pmela-page-R | CCCCCTTGGTAAGGTTTCCT | F1 and F2 mutant fish screening |
| pmelb-page-F | ATTGTCCTGAATGAAGTATT | F1 and F2 mutant fish screening |
| pmelb-page-R | AGGTCAATAGTGAAGGTAACT | F1 and F2 mutant fish screening |
| pmela-RT-PCR-F | ATTGGACCGGAGTTTTCCCC | Validation of pmela expression |
| pmela-RT-PCR-R | GAGGCAACTACCACGACCTC | Validation of pmela expression |
| pmelb-RT-PCR-F | ACCCCTCATGGAACACCAAG | Validation of pmelb expression |
| pmelb-RT-PCR-R | CCCGAGCTGATGTAGGTGTG | Validation of pmelb expression |
| β-actin-F | GGCATCACACCTTCTACAACGA | Internal control |
| β-actin-R | ACGCTCTGTCAGGATCTTCA | Internal control |
| M13+ | CGCCAGGGTTTTCCCAGTCACG | Sequencing and clone screening |
| M13- | AGCGGATAACAATTTCACACAG | Sequencing and clone screening |
| Primer . | Sequence (5ʹ–3ʹ) . | Purpose . |
|---|---|---|
| pmela-Cas9-F | CTTGACCTCTCCTTCCACATTTGAC | Positive gene knockout fish screening |
| pmela-Cas9-R | TGCTGCTAAGCTCTTATGGGG | Positive gene knockout fish screening |
| pmelb-Cas9-F | CCAAAAACCGCTTCACTCGTT | Positive gene knockout fish screening |
| pmelb-Cas9-R | TCTTCCTGTGGGGACTGACC | Positive gene knockout fish screening |
| pmela-page-F | AGCAAAAGCGGGCTTCACTA | F1 and F2 mutant fish screening |
| pmela-page-R | CCCCCTTGGTAAGGTTTCCT | F1 and F2 mutant fish screening |
| pmelb-page-F | ATTGTCCTGAATGAAGTATT | F1 and F2 mutant fish screening |
| pmelb-page-R | AGGTCAATAGTGAAGGTAACT | F1 and F2 mutant fish screening |
| pmela-RT-PCR-F | ATTGGACCGGAGTTTTCCCC | Validation of pmela expression |
| pmela-RT-PCR-R | GAGGCAACTACCACGACCTC | Validation of pmela expression |
| pmelb-RT-PCR-F | ACCCCTCATGGAACACCAAG | Validation of pmelb expression |
| pmelb-RT-PCR-R | CCCGAGCTGATGTAGGTGTG | Validation of pmelb expression |
| β-actin-F | GGCATCACACCTTCTACAACGA | Internal control |
| β-actin-R | ACGCTCTGTCAGGATCTTCA | Internal control |
| M13+ | CGCCAGGGTTTTCCCAGTCACG | Sequencing and clone screening |
| M13- | AGCGGATAACAATTTCACACAG | Sequencing and clone screening |
| Primer . | Sequence (5ʹ–3ʹ) . | Purpose . |
|---|---|---|
| pmela-Cas9-F | CTTGACCTCTCCTTCCACATTTGAC | Positive gene knockout fish screening |
| pmela-Cas9-R | TGCTGCTAAGCTCTTATGGGG | Positive gene knockout fish screening |
| pmelb-Cas9-F | CCAAAAACCGCTTCACTCGTT | Positive gene knockout fish screening |
| pmelb-Cas9-R | TCTTCCTGTGGGGACTGACC | Positive gene knockout fish screening |
| pmela-page-F | AGCAAAAGCGGGCTTCACTA | F1 and F2 mutant fish screening |
| pmela-page-R | CCCCCTTGGTAAGGTTTCCT | F1 and F2 mutant fish screening |
| pmelb-page-F | ATTGTCCTGAATGAAGTATT | F1 and F2 mutant fish screening |
| pmelb-page-R | AGGTCAATAGTGAAGGTAACT | F1 and F2 mutant fish screening |
| pmela-RT-PCR-F | ATTGGACCGGAGTTTTCCCC | Validation of pmela expression |
| pmela-RT-PCR-R | GAGGCAACTACCACGACCTC | Validation of pmela expression |
| pmelb-RT-PCR-F | ACCCCTCATGGAACACCAAG | Validation of pmelb expression |
| pmelb-RT-PCR-R | CCCGAGCTGATGTAGGTGTG | Validation of pmelb expression |
| β-actin-F | GGCATCACACCTTCTACAACGA | Internal control |
| β-actin-R | ACGCTCTGTCAGGATCTTCA | Internal control |
| M13+ | CGCCAGGGTTTTCCCAGTCACG | Sequencing and clone screening |
| M13- | AGCGGATAACAATTTCACACAG | Sequencing and clone screening |
| Primer . | Sequence (5ʹ–3ʹ) . | Purpose . |
|---|---|---|
| pmela-Cas9-F | CTTGACCTCTCCTTCCACATTTGAC | Positive gene knockout fish screening |
| pmela-Cas9-R | TGCTGCTAAGCTCTTATGGGG | Positive gene knockout fish screening |
| pmelb-Cas9-F | CCAAAAACCGCTTCACTCGTT | Positive gene knockout fish screening |
| pmelb-Cas9-R | TCTTCCTGTGGGGACTGACC | Positive gene knockout fish screening |
| pmela-page-F | AGCAAAAGCGGGCTTCACTA | F1 and F2 mutant fish screening |
| pmela-page-R | CCCCCTTGGTAAGGTTTCCT | F1 and F2 mutant fish screening |
| pmelb-page-F | ATTGTCCTGAATGAAGTATT | F1 and F2 mutant fish screening |
| pmelb-page-R | AGGTCAATAGTGAAGGTAACT | F1 and F2 mutant fish screening |
| pmela-RT-PCR-F | ATTGGACCGGAGTTTTCCCC | Validation of pmela expression |
| pmela-RT-PCR-R | GAGGCAACTACCACGACCTC | Validation of pmela expression |
| pmelb-RT-PCR-F | ACCCCTCATGGAACACCAAG | Validation of pmelb expression |
| pmelb-RT-PCR-R | CCCGAGCTGATGTAGGTGTG | Validation of pmelb expression |
| β-actin-F | GGCATCACACCTTCTACAACGA | Internal control |
| β-actin-R | ACGCTCTGTCAGGATCTTCA | Internal control |
| M13+ | CGCCAGGGTTTTCCCAGTCACG | Sequencing and clone screening |
| M13- | AGCGGATAACAATTTCACACAG | Sequencing and clone screening |
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