Primer sequences for white sturgeon (A. transmontanus), hsp70, hsp90a, hsp90b, hsp47, hif1a, hb-a, hb-b, hxk, pepck-c, pepck-m, g6p, igf2, rps5, rps8. Target genes were chosen based on their roles in the response to temperature stress, hypoxia, energy allocation and growth. rps5 and rps8 were used as reference genes because they showed stable expression across all treatments. Efficiencies are listed as a percentage (%)
| Gene name . | Gene category . | Forward primer . | Reverse primer . | Efficiency (%) . |
|---|---|---|---|---|
| hsp70 | Temperature | CCATGAACCCCAGCAACACT | TGCACAACAGAGTCGTCGTA | 100.8 |
| hsp90a | Temperature | CCTTGATTGCCTCCTCTGTT | GACTCATTCCAACCGCATCTA | 103.4 |
| hsp90b | Temperature | ACTTGGTCCTTGCTCTCACC | GCGATACCACAGCTCTCAGT | 104.9 |
| hsp47 | Temperature | ACCTGTAATAAGTCCGCATCTC | GTGTACGAACCACCCAAGAA | 96.5 |
| hif1a | Hypoxia | GCATCTGAGGATAGTGGTAAAG | CTGTTGGCAGTAGGAGAATG | 99.6 |
| hb-a | Blood oxygenation | CGAATTGTCACCAGGTTCTAT | GTGAGAGGCATCTTTGTAGTT | 105.4 |
| hb-b | Blood oxygenation | GCTTGCACCAGGGATTT | ACTAAACACCAGCCATCTTAC | 104.7 |
| hxk | Energy allocation | GAAGCCGCCAGAACAATAA | GTACACCAAGACCCACTTTAG | 100.2 |
| pepck-c | Energy allocation | CGTATGCCCTGACCTTAATC | CGACAATAACTTGAGACACAATC | 100.5 |
| pepck-m | Energy allocation | CAGTCAGCGAGTTCGTTTC | CTTTCCAGTGTTCCCAGTATC | 97.5 |
| g6p | Energy allocation | CGCTCTGCTTCTCCAATAG | CCCTAACAACCTCACACTAAA | 98.9 |
| igf2 | Growth | GGAGAATTACACCAGCAAGAA | CACAGAAAGGACGCCAATAA | 95.1 |
| gh1 | Growth | CGACCGAGTGTTTGAGAAA | AGTGAGCTTCAGCAAAGTAG | 104.9 |
| rps5 | Reference | ACTCGACCCGAATTGGACG | GTTGACTCTACGCAGGGGG | 99.9 |
| rps8 | Reference | GGGCGACCCAATTCATACTT | CCAGGGACAACTGGCATAAA | 102.7 |
| Gene name . | Gene category . | Forward primer . | Reverse primer . | Efficiency (%) . |
|---|---|---|---|---|
| hsp70 | Temperature | CCATGAACCCCAGCAACACT | TGCACAACAGAGTCGTCGTA | 100.8 |
| hsp90a | Temperature | CCTTGATTGCCTCCTCTGTT | GACTCATTCCAACCGCATCTA | 103.4 |
| hsp90b | Temperature | ACTTGGTCCTTGCTCTCACC | GCGATACCACAGCTCTCAGT | 104.9 |
| hsp47 | Temperature | ACCTGTAATAAGTCCGCATCTC | GTGTACGAACCACCCAAGAA | 96.5 |
| hif1a | Hypoxia | GCATCTGAGGATAGTGGTAAAG | CTGTTGGCAGTAGGAGAATG | 99.6 |
| hb-a | Blood oxygenation | CGAATTGTCACCAGGTTCTAT | GTGAGAGGCATCTTTGTAGTT | 105.4 |
| hb-b | Blood oxygenation | GCTTGCACCAGGGATTT | ACTAAACACCAGCCATCTTAC | 104.7 |
| hxk | Energy allocation | GAAGCCGCCAGAACAATAA | GTACACCAAGACCCACTTTAG | 100.2 |
| pepck-c | Energy allocation | CGTATGCCCTGACCTTAATC | CGACAATAACTTGAGACACAATC | 100.5 |
| pepck-m | Energy allocation | CAGTCAGCGAGTTCGTTTC | CTTTCCAGTGTTCCCAGTATC | 97.5 |
| g6p | Energy allocation | CGCTCTGCTTCTCCAATAG | CCCTAACAACCTCACACTAAA | 98.9 |
| igf2 | Growth | GGAGAATTACACCAGCAAGAA | CACAGAAAGGACGCCAATAA | 95.1 |
| gh1 | Growth | CGACCGAGTGTTTGAGAAA | AGTGAGCTTCAGCAAAGTAG | 104.9 |
| rps5 | Reference | ACTCGACCCGAATTGGACG | GTTGACTCTACGCAGGGGG | 99.9 |
| rps8 | Reference | GGGCGACCCAATTCATACTT | CCAGGGACAACTGGCATAAA | 102.7 |
Primer sequences for white sturgeon (A. transmontanus), hsp70, hsp90a, hsp90b, hsp47, hif1a, hb-a, hb-b, hxk, pepck-c, pepck-m, g6p, igf2, rps5, rps8. Target genes were chosen based on their roles in the response to temperature stress, hypoxia, energy allocation and growth. rps5 and rps8 were used as reference genes because they showed stable expression across all treatments. Efficiencies are listed as a percentage (%)
| Gene name . | Gene category . | Forward primer . | Reverse primer . | Efficiency (%) . |
|---|---|---|---|---|
| hsp70 | Temperature | CCATGAACCCCAGCAACACT | TGCACAACAGAGTCGTCGTA | 100.8 |
| hsp90a | Temperature | CCTTGATTGCCTCCTCTGTT | GACTCATTCCAACCGCATCTA | 103.4 |
| hsp90b | Temperature | ACTTGGTCCTTGCTCTCACC | GCGATACCACAGCTCTCAGT | 104.9 |
| hsp47 | Temperature | ACCTGTAATAAGTCCGCATCTC | GTGTACGAACCACCCAAGAA | 96.5 |
| hif1a | Hypoxia | GCATCTGAGGATAGTGGTAAAG | CTGTTGGCAGTAGGAGAATG | 99.6 |
| hb-a | Blood oxygenation | CGAATTGTCACCAGGTTCTAT | GTGAGAGGCATCTTTGTAGTT | 105.4 |
| hb-b | Blood oxygenation | GCTTGCACCAGGGATTT | ACTAAACACCAGCCATCTTAC | 104.7 |
| hxk | Energy allocation | GAAGCCGCCAGAACAATAA | GTACACCAAGACCCACTTTAG | 100.2 |
| pepck-c | Energy allocation | CGTATGCCCTGACCTTAATC | CGACAATAACTTGAGACACAATC | 100.5 |
| pepck-m | Energy allocation | CAGTCAGCGAGTTCGTTTC | CTTTCCAGTGTTCCCAGTATC | 97.5 |
| g6p | Energy allocation | CGCTCTGCTTCTCCAATAG | CCCTAACAACCTCACACTAAA | 98.9 |
| igf2 | Growth | GGAGAATTACACCAGCAAGAA | CACAGAAAGGACGCCAATAA | 95.1 |
| gh1 | Growth | CGACCGAGTGTTTGAGAAA | AGTGAGCTTCAGCAAAGTAG | 104.9 |
| rps5 | Reference | ACTCGACCCGAATTGGACG | GTTGACTCTACGCAGGGGG | 99.9 |
| rps8 | Reference | GGGCGACCCAATTCATACTT | CCAGGGACAACTGGCATAAA | 102.7 |
| Gene name . | Gene category . | Forward primer . | Reverse primer . | Efficiency (%) . |
|---|---|---|---|---|
| hsp70 | Temperature | CCATGAACCCCAGCAACACT | TGCACAACAGAGTCGTCGTA | 100.8 |
| hsp90a | Temperature | CCTTGATTGCCTCCTCTGTT | GACTCATTCCAACCGCATCTA | 103.4 |
| hsp90b | Temperature | ACTTGGTCCTTGCTCTCACC | GCGATACCACAGCTCTCAGT | 104.9 |
| hsp47 | Temperature | ACCTGTAATAAGTCCGCATCTC | GTGTACGAACCACCCAAGAA | 96.5 |
| hif1a | Hypoxia | GCATCTGAGGATAGTGGTAAAG | CTGTTGGCAGTAGGAGAATG | 99.6 |
| hb-a | Blood oxygenation | CGAATTGTCACCAGGTTCTAT | GTGAGAGGCATCTTTGTAGTT | 105.4 |
| hb-b | Blood oxygenation | GCTTGCACCAGGGATTT | ACTAAACACCAGCCATCTTAC | 104.7 |
| hxk | Energy allocation | GAAGCCGCCAGAACAATAA | GTACACCAAGACCCACTTTAG | 100.2 |
| pepck-c | Energy allocation | CGTATGCCCTGACCTTAATC | CGACAATAACTTGAGACACAATC | 100.5 |
| pepck-m | Energy allocation | CAGTCAGCGAGTTCGTTTC | CTTTCCAGTGTTCCCAGTATC | 97.5 |
| g6p | Energy allocation | CGCTCTGCTTCTCCAATAG | CCCTAACAACCTCACACTAAA | 98.9 |
| igf2 | Growth | GGAGAATTACACCAGCAAGAA | CACAGAAAGGACGCCAATAA | 95.1 |
| gh1 | Growth | CGACCGAGTGTTTGAGAAA | AGTGAGCTTCAGCAAAGTAG | 104.9 |
| rps5 | Reference | ACTCGACCCGAATTGGACG | GTTGACTCTACGCAGGGGG | 99.9 |
| rps8 | Reference | GGGCGACCCAATTCATACTT | CCAGGGACAACTGGCATAAA | 102.7 |
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