| Endpoint of interest | Assay proposed |
| High‐throughput transcriptomics | Metabolically competent human liver cells/MCF‐7 (Tempo‐Seq®) |
| Hepatotoxicity | 2D HepaRG® cells |
| Developmental toxicity | Zebrafish embryo assay |
| Developmental neurotoxicity | Multielectrode array in neonatal cortical cells and neurite outgrowth |
| Immunotoxicity | Cytokine alterations in human vascular endothelial cells (BioSeek®) |
| Hepatic clearance | Metabolic clearance in 50 donor‐pooled hepatocyte suspensions |
| Plasma protein binding | Serum protein binding assay using human serum |
| Enterohepatic recirculation | Qualyst B‐CLEAR® hepatocyte transporter assay |
| In vitro disposition | In vitro disposition in cell lines under study |
| Endpoint of interest | Assay proposed |
| High‐throughput transcriptomics | Metabolically competent human liver cells/MCF‐7 (Tempo‐Seq®) |
| Hepatotoxicity | 2D HepaRG® cells |
| Developmental toxicity | Zebrafish embryo assay |
| Developmental neurotoxicity | Multielectrode array in neonatal cortical cells and neurite outgrowth |
| Immunotoxicity | Cytokine alterations in human vascular endothelial cells (BioSeek®) |
| Hepatic clearance | Metabolic clearance in 50 donor‐pooled hepatocyte suspensions |
| Plasma protein binding | Serum protein binding assay using human serum |
| Enterohepatic recirculation | Qualyst B‐CLEAR® hepatocyte transporter assay |
| In vitro disposition | In vitro disposition in cell lines under study |
REACT = Responsive Evaluation and Assessment of Chemical Toxicity.
| Endpoint of interest | Assay proposed |
| High‐throughput transcriptomics | Metabolically competent human liver cells/MCF‐7 (Tempo‐Seq®) |
| Hepatotoxicity | 2D HepaRG® cells |
| Developmental toxicity | Zebrafish embryo assay |
| Developmental neurotoxicity | Multielectrode array in neonatal cortical cells and neurite outgrowth |
| Immunotoxicity | Cytokine alterations in human vascular endothelial cells (BioSeek®) |
| Hepatic clearance | Metabolic clearance in 50 donor‐pooled hepatocyte suspensions |
| Plasma protein binding | Serum protein binding assay using human serum |
| Enterohepatic recirculation | Qualyst B‐CLEAR® hepatocyte transporter assay |
| In vitro disposition | In vitro disposition in cell lines under study |
| Endpoint of interest | Assay proposed |
| High‐throughput transcriptomics | Metabolically competent human liver cells/MCF‐7 (Tempo‐Seq®) |
| Hepatotoxicity | 2D HepaRG® cells |
| Developmental toxicity | Zebrafish embryo assay |
| Developmental neurotoxicity | Multielectrode array in neonatal cortical cells and neurite outgrowth |
| Immunotoxicity | Cytokine alterations in human vascular endothelial cells (BioSeek®) |
| Hepatic clearance | Metabolic clearance in 50 donor‐pooled hepatocyte suspensions |
| Plasma protein binding | Serum protein binding assay using human serum |
| Enterohepatic recirculation | Qualyst B‐CLEAR® hepatocyte transporter assay |
| In vitro disposition | In vitro disposition in cell lines under study |
REACT = Responsive Evaluation and Assessment of Chemical Toxicity.
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