SDS-PAGE . | non βME x103 . | plus βME x103 . | βME reduction . | t-test . |
---|---|---|---|---|
Rat 0.08 µg Se/g | 761 ± 140 | 873 ± 29 | −15% | 0.384 |
Rat 5.0 µg Se/g | 3397 ± 400 | 1210 ± 39 | 64% | 0.028 |
Turkey 0.4 µg Se/g | 726 ± 173 | 727 ± 31 | 0% | 0.765 |
Turkey 5.0 µg Se/g | 2569 ± 475 | 938 ± 23 | 63% | 0.024 |
Native-PAGE | nonβME x103 | plusβME x103 | βME reduction | t-test |
Rat 0.08 µg Se/g | 783 ± 148 | 647 ± 416 | 17% | 0.945 |
Rat 5.0 µg Se/g | 1870 ± 290 | 1049 ± 54 | 44% | 0.032 |
Turkey 0.4 µg Se/g | 658 ± 20 | 775 ± 60 | −18% | 0.056 |
Turkey 5.0 µg Se/g | 2089 ± 203 | 647 ± 95 | 69% | 0.013 |
SDS-PAGE . | non βME x103 . | plus βME x103 . | βME reduction . | t-test . |
---|---|---|---|---|
Rat 0.08 µg Se/g | 761 ± 140 | 873 ± 29 | −15% | 0.384 |
Rat 5.0 µg Se/g | 3397 ± 400 | 1210 ± 39 | 64% | 0.028 |
Turkey 0.4 µg Se/g | 726 ± 173 | 727 ± 31 | 0% | 0.765 |
Turkey 5.0 µg Se/g | 2569 ± 475 | 938 ± 23 | 63% | 0.024 |
Native-PAGE | nonβME x103 | plusβME x103 | βME reduction | t-test |
Rat 0.08 µg Se/g | 783 ± 148 | 647 ± 416 | 17% | 0.945 |
Rat 5.0 µg Se/g | 1870 ± 290 | 1049 ± 54 | 44% | 0.032 |
Turkey 0.4 µg Se/g | 658 ± 20 | 775 ± 60 | −18% | 0.056 |
Turkey 5.0 µg Se/g | 2089 ± 203 | 647 ± 95 | 69% | 0.013 |
Cumulative 78Se cpm recovery in LA-ICP-MS analysis of individual replicates (n = 3; Mean ± SEM). βME reduction (or 78Se lost) is % of total non-βME cpm that were not present in the plus-βME cpm; a negative βME reduction indicates that more 78Se was recovered in the plus-βME than in non-βME profiles.
SDS-PAGE . | non βME x103 . | plus βME x103 . | βME reduction . | t-test . |
---|---|---|---|---|
Rat 0.08 µg Se/g | 761 ± 140 | 873 ± 29 | −15% | 0.384 |
Rat 5.0 µg Se/g | 3397 ± 400 | 1210 ± 39 | 64% | 0.028 |
Turkey 0.4 µg Se/g | 726 ± 173 | 727 ± 31 | 0% | 0.765 |
Turkey 5.0 µg Se/g | 2569 ± 475 | 938 ± 23 | 63% | 0.024 |
Native-PAGE | nonβME x103 | plusβME x103 | βME reduction | t-test |
Rat 0.08 µg Se/g | 783 ± 148 | 647 ± 416 | 17% | 0.945 |
Rat 5.0 µg Se/g | 1870 ± 290 | 1049 ± 54 | 44% | 0.032 |
Turkey 0.4 µg Se/g | 658 ± 20 | 775 ± 60 | −18% | 0.056 |
Turkey 5.0 µg Se/g | 2089 ± 203 | 647 ± 95 | 69% | 0.013 |
SDS-PAGE . | non βME x103 . | plus βME x103 . | βME reduction . | t-test . |
---|---|---|---|---|
Rat 0.08 µg Se/g | 761 ± 140 | 873 ± 29 | −15% | 0.384 |
Rat 5.0 µg Se/g | 3397 ± 400 | 1210 ± 39 | 64% | 0.028 |
Turkey 0.4 µg Se/g | 726 ± 173 | 727 ± 31 | 0% | 0.765 |
Turkey 5.0 µg Se/g | 2569 ± 475 | 938 ± 23 | 63% | 0.024 |
Native-PAGE | nonβME x103 | plusβME x103 | βME reduction | t-test |
Rat 0.08 µg Se/g | 783 ± 148 | 647 ± 416 | 17% | 0.945 |
Rat 5.0 µg Se/g | 1870 ± 290 | 1049 ± 54 | 44% | 0.032 |
Turkey 0.4 µg Se/g | 658 ± 20 | 775 ± 60 | −18% | 0.056 |
Turkey 5.0 µg Se/g | 2089 ± 203 | 647 ± 95 | 69% | 0.013 |
Cumulative 78Se cpm recovery in LA-ICP-MS analysis of individual replicates (n = 3; Mean ± SEM). βME reduction (or 78Se lost) is % of total non-βME cpm that were not present in the plus-βME cpm; a negative βME reduction indicates that more 78Se was recovered in the plus-βME than in non-βME profiles.
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