Table 2. Open in new tab RNA affinities...

	RBDs	cores	K_d (μM) (32mer)	K_d (μM) (32/9mer)	K_d (μM) (32mer)	K_d (μM) (32/9mer)
# of RBDs
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
Hera/Hera_1–419	1	2	0.25 ± 0.09	3.0 ± 0.3	0.50 ± 0.18	6.0 ± 0.6
					0.5 ± 0.18	6.0 ± 0.6
Hera_1–419	0	2	16 ± 7	11 ± 5	32 ± 14	22 ± 10
					34 ± 15	23 ± 11

# of (functional) cores
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
Hera/Hera_K51Q	2	1	0.08 ± 0.02	2.5 ± 0.4	0.16 ± 0.04	5.0 ± 0.8
					0.20 ± 0.072	5.0 ± 0.82
Hera_K51Q	2	0	0.17 ± 0.02	2.1 ± 0.4	0.34 ± 0.04	4.2 ± 0.8
					0.36 ± 0.042	4.3 ± 0.84
Hera_1–419	0	2	16 ± 7	11 ± 5	32 ± 14	22 ± 10
					34 ± 15	23 ± 11
Hera_1–365	0	1	81 ± 29	n.d.^b	81 ± 29	n.d.

position of RBD
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
cis	1	1	0.126 ± 0.009	1.4 ± 0.5	0.126 ± 0.009	1.4 ± 0.5
trans	1	1	3.1 ± 0.5	16 ± 6	3.1 ± 0.5	16 ± 6
cis-like	1	1	n.d.	n.d.	n.d.	n.d.
trans-like	1	1	n.d.	n.d.	n.d.	n.d.
RBD	1	0	1.0 ± 0.2	17 ± 3	1.0 ± 0.2	17 ± 3

RBDs

cores

K_d (μM) (32mer)

K_d (μM) (32/9mer)

K_d (μM) (32mer)

K_d (μM) (32/9mer)

per protomer^a

# of RBDs

Hera

0.06 ± 0.01

2.0 ± 0.3

0.11 ± 0.02

4.0 ± 0.6

0.13 ± 0.02

4.1 ± 0.63

Hera/Hera_1–419

0.25 ± 0.09

3.0 ± 0.3

0.50 ± 0.18

6.0 ± 0.6

0.5 ± 0.18

6.0 ± 0.6

Hera_1–419

16 ± 7

11 ± 5

32 ± 14

22 ± 10

34 ± 15

23 ± 11

# of (functional) cores

Hera

0.06 ± 0.01

2.0 ± 0.3

0.11 ± 0.02

4.0 ± 0.6

0.13 ± 0.02

4.1 ± 0.63

Hera/Hera_K51Q

0.08 ± 0.02

2.5 ± 0.4

0.16 ± 0.04

5.0 ± 0.8

0.20 ± 0.072

5.0 ± 0.82

Hera_K51Q

0.17 ± 0.02

2.1 ± 0.4

0.34 ± 0.04

4.2 ± 0.8

0.36 ± 0.042

4.3 ± 0.84

Hera_1–419

16 ± 7

11 ± 5

32 ± 14

22 ± 10

34 ± 15

23 ± 11

Hera_1–365

81 ± 29

n.d.^b

81 ± 29

n.d.

position of RBD

Hera

0.06 ± 0.01

2.0 ± 0.3

0.11 ± 0.02

4.0 ± 0.6

0.13 ± 0.02

4.1 ± 0.63

cis

0.126 ± 0.009

1.4 ± 0.5

0.126 ± 0.009

1.4 ± 0.5

trans

3.1 ± 0.5

16 ± 6

3.1 ± 0.5

16 ± 6

cis-like

n.d.

trans-like

n.d.

RBD

1.0 ± 0.2

17 ± 3

1.0 ± 0.2

17 ± 3

	RBDs	cores	K_d (μM) (32mer)	K_d (μM) (32/9mer)	K_d (μM) (32mer)	K_d (μM) (32/9mer)
# of RBDs
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
Hera/Hera_1–419	1	2	0.25 ± 0.09	3.0 ± 0.3	0.50 ± 0.18	6.0 ± 0.6
					0.5 ± 0.18	6.0 ± 0.6
Hera_1–419	0	2	16 ± 7	11 ± 5	32 ± 14	22 ± 10
					34 ± 15	23 ± 11

# of (functional) cores
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
Hera/Hera_K51Q	2	1	0.08 ± 0.02	2.5 ± 0.4	0.16 ± 0.04	5.0 ± 0.8
					0.20 ± 0.072	5.0 ± 0.82
Hera_K51Q	2	0	0.17 ± 0.02	2.1 ± 0.4	0.34 ± 0.04	4.2 ± 0.8
					0.36 ± 0.042	4.3 ± 0.84
Hera_1–419	0	2	16 ± 7	11 ± 5	32 ± 14	22 ± 10
					34 ± 15	23 ± 11
Hera_1–365	0	1	81 ± 29	n.d.^b	81 ± 29	n.d.

position of RBD
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
cis	1	1	0.126 ± 0.009	1.4 ± 0.5	0.126 ± 0.009	1.4 ± 0.5
trans	1	1	3.1 ± 0.5	16 ± 6	3.1 ± 0.5	16 ± 6
cis-like	1	1	n.d.	n.d.	n.d.	n.d.
trans-like	1	1	n.d.	n.d.	n.d.	n.d.
RBD	1	0	1.0 ± 0.2	17 ± 3	1.0 ± 0.2	17 ± 3

Table 2.

Open in new tab

RNA affinities

	RBDs	cores	K_d (μM) (32mer)	K_d (μM) (32/9mer)	K_d (μM) (32mer)	K_d (μM) (32/9mer)
					per protomer^a
# of RBDs
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
Hera/Hera_1–419	1	2	0.25 ± 0.09	3.0 ± 0.3	0.50 ± 0.18	6.0 ± 0.6
					0.5 ± 0.18	6.0 ± 0.6
Hera_1–419	0	2	16 ± 7	11 ± 5	32 ± 14	22 ± 10
					34 ± 15	23 ± 11

# of (functional) cores
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
Hera/Hera_K51Q	2	1	0.08 ± 0.02	2.5 ± 0.4	0.16 ± 0.04	5.0 ± 0.8
					0.20 ± 0.072	5.0 ± 0.82
Hera_K51Q	2	0	0.17 ± 0.02	2.1 ± 0.4	0.34 ± 0.04	4.2 ± 0.8
					0.36 ± 0.042	4.3 ± 0.84
Hera_1–419	0	2	16 ± 7	11 ± 5	32 ± 14	22 ± 10
					34 ± 15	23 ± 11
Hera_1–365	0	1	81 ± 29	n.d.^b	81 ± 29	n.d.

position of RBD
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
cis	1	1	0.126 ± 0.009	1.4 ± 0.5	0.126 ± 0.009	1.4 ± 0.5
trans	1	1	3.1 ± 0.5	16 ± 6	3.1 ± 0.5	16 ± 6
cis-like	1	1	n.d.	n.d.	n.d.	n.d.
trans-like	1	1	n.d.	n.d.	n.d.	n.d.
RBD	1	0	1.0 ± 0.2	17 ± 3	1.0 ± 0.2	17 ± 3

	RBDs	cores	K_d (μM) (32mer)	K_d (μM) (32/9mer)	K_d (μM) (32mer)	K_d (μM) (32/9mer)
					per protomer^a
# of RBDs
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
Hera/Hera_1–419	1	2	0.25 ± 0.09	3.0 ± 0.3	0.50 ± 0.18	6.0 ± 0.6
					0.5 ± 0.18	6.0 ± 0.6
Hera_1–419	0	2	16 ± 7	11 ± 5	32 ± 14	22 ± 10
					34 ± 15	23 ± 11

# of (functional) cores
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
Hera/Hera_K51Q	2	1	0.08 ± 0.02	2.5 ± 0.4	0.16 ± 0.04	5.0 ± 0.8
					0.20 ± 0.072	5.0 ± 0.82
Hera_K51Q	2	0	0.17 ± 0.02	2.1 ± 0.4	0.34 ± 0.04	4.2 ± 0.8
					0.36 ± 0.042	4.3 ± 0.84
Hera_1–419	0	2	16 ± 7	11 ± 5	32 ± 14	22 ± 10
					34 ± 15	23 ± 11
Hera_1–365	0	1	81 ± 29	n.d.^b	81 ± 29	n.d.

position of RBD
Hera	2	2	0.06 ± 0.01	2.0 ± 0.3	0.11 ± 0.02	4.0 ± 0.6
					0.13 ± 0.02	4.1 ± 0.63
cis	1	1	0.126 ± 0.009	1.4 ± 0.5	0.126 ± 0.009	1.4 ± 0.5
trans	1	1	3.1 ± 0.5	16 ± 6	3.1 ± 0.5	16 ± 6
cis-like	1	1	n.d.	n.d.	n.d.	n.d.
trans-like	1	1	n.d.	n.d.	n.d.	n.d.
RBD	1	0	1.0 ± 0.2	17 ± 3	1.0 ± 0.2	17 ± 3

Values were determined from analyses of anisotropy titrations using equation (4) (upper row) or from numerical analyses with Dynafit (bottom row, see text).

^aAs Hera concentrations are given as dimer concentrations, the K_d values determined here reflect the values for both protomers. For homodimers, the true K_d value per protomer is obtained by multiplying K_d by two; for heterodimers, formed by protomers with different RNA affinities, multiplying the value by two gives an apparent K_d value per protomer.

^bn.d.: not determined

Errors are the errors of the concatenated fit of the cumulative data from at least two independent experiments.

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