| Material . | Method . | Study summary . | Reference . |
|---|---|---|---|
| canine osteosarcoma samples | RT-PCR | GH present in 25% of samples | (324) |
| rat osteosarcoma cells + GH | immunoprecipitation, Western blot | GH activates JAK2-STAT5 | (325) |
| patient samples | IHC | GHR overexpression in osteosarcoma samples | (326) |
| OS cell lines, nude mice xenograft | GHR knockdown | attenuated cell growth, increased apoptosis, suppressed xenograft growth | (326) |
| patient samples (OS and Ewing sarcoma) | RT-PCR and ELISA, clinical correlation | increased serum IGF1, IGFBP3 correlate with tumor grade and relapse | (327) |
| Material . | Method . | Study summary . | Reference . |
|---|---|---|---|
| canine osteosarcoma samples | RT-PCR | GH present in 25% of samples | (324) |
| rat osteosarcoma cells + GH | immunoprecipitation, Western blot | GH activates JAK2-STAT5 | (325) |
| patient samples | IHC | GHR overexpression in osteosarcoma samples | (326) |
| OS cell lines, nude mice xenograft | GHR knockdown | attenuated cell growth, increased apoptosis, suppressed xenograft growth | (326) |
| patient samples (OS and Ewing sarcoma) | RT-PCR and ELISA, clinical correlation | increased serum IGF1, IGFBP3 correlate with tumor grade and relapse | (327) |
Abbreviations: GH, growth hormone; GHR, growth hormone receptor; IGF1, insulin-like growth factor 1; IGFBP, insulin-like growth factor binding protein.
| Material . | Method . | Study summary . | Reference . |
|---|---|---|---|
| canine osteosarcoma samples | RT-PCR | GH present in 25% of samples | (324) |
| rat osteosarcoma cells + GH | immunoprecipitation, Western blot | GH activates JAK2-STAT5 | (325) |
| patient samples | IHC | GHR overexpression in osteosarcoma samples | (326) |
| OS cell lines, nude mice xenograft | GHR knockdown | attenuated cell growth, increased apoptosis, suppressed xenograft growth | (326) |
| patient samples (OS and Ewing sarcoma) | RT-PCR and ELISA, clinical correlation | increased serum IGF1, IGFBP3 correlate with tumor grade and relapse | (327) |
| Material . | Method . | Study summary . | Reference . |
|---|---|---|---|
| canine osteosarcoma samples | RT-PCR | GH present in 25% of samples | (324) |
| rat osteosarcoma cells + GH | immunoprecipitation, Western blot | GH activates JAK2-STAT5 | (325) |
| patient samples | IHC | GHR overexpression in osteosarcoma samples | (326) |
| OS cell lines, nude mice xenograft | GHR knockdown | attenuated cell growth, increased apoptosis, suppressed xenograft growth | (326) |
| patient samples (OS and Ewing sarcoma) | RT-PCR and ELISA, clinical correlation | increased serum IGF1, IGFBP3 correlate with tumor grade and relapse | (327) |
Abbreviations: GH, growth hormone; GHR, growth hormone receptor; IGF1, insulin-like growth factor 1; IGFBP, insulin-like growth factor binding protein.
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