Table 2.
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Linearity, imprecision (within and between day) on low and high level, LLOQ, recovery, and ion suppression for free 21DF and free cortisol measured by LC-MS/MS after dialysis

 Free 21DFFree cortisol
Linearity
Linearity range (nmol/L)0.13-99.20.08-66.0
Deviation from linearity (%)1.550.96
Imprecision low
Concentration (nmol/L)0.3611.4
Within day (%)2.82.6
Between day (%)8.54.6
Imprecision high
Concentration (nmol/L)4.89104.4
Within day (%)3.32.5
Between day (%)2.84.0
LLOQ (nmol/L)0.19<0.32a
Recovery
Concentration (nmol/L)29.941.496
Average recovery (%)10897.3
Ion suppression (%)<1<1
 Free 21DFFree cortisol
Linearity
Linearity range (nmol/L)0.13-99.20.08-66.0
Deviation from linearity (%)1.550.96
Imprecision low
Concentration (nmol/L)0.3611.4
Within day (%)2.82.6
Between day (%)8.54.6
Imprecision high
Concentration (nmol/L)4.89104.4
Within day (%)3.32.5
Between day (%)2.84.0
LLOQ (nmol/L)0.19<0.32a
Recovery
Concentration (nmol/L)29.941.496
Average recovery (%)10897.3
Ion suppression (%)<1<1

Abbreviations: 21DF, 21-deoxycortisol; LC-MS/MS, liquid chromatography tandem mass spectrometry; LLOQ, lower limit of quantification.

aMean coefficient of variation of 6.1%.

Table 2.
Open in new tab

Linearity, imprecision (within and between day) on low and high level, LLOQ, recovery, and ion suppression for free 21DF and free cortisol measured by LC-MS/MS after dialysis

 Free 21DFFree cortisol
Linearity
Linearity range (nmol/L)0.13-99.20.08-66.0
Deviation from linearity (%)1.550.96
Imprecision low
Concentration (nmol/L)0.3611.4
Within day (%)2.82.6
Between day (%)8.54.6
Imprecision high
Concentration (nmol/L)4.89104.4
Within day (%)3.32.5
Between day (%)2.84.0
LLOQ (nmol/L)0.19<0.32a
Recovery
Concentration (nmol/L)29.941.496
Average recovery (%)10897.3
Ion suppression (%)<1<1
 Free 21DFFree cortisol
Linearity
Linearity range (nmol/L)0.13-99.20.08-66.0
Deviation from linearity (%)1.550.96
Imprecision low
Concentration (nmol/L)0.3611.4
Within day (%)2.82.6
Between day (%)8.54.6
Imprecision high
Concentration (nmol/L)4.89104.4
Within day (%)3.32.5
Between day (%)2.84.0
LLOQ (nmol/L)0.19<0.32a
Recovery
Concentration (nmol/L)29.941.496
Average recovery (%)10897.3
Ion suppression (%)<1<1

Abbreviations: 21DF, 21-deoxycortisol; LC-MS/MS, liquid chromatography tandem mass spectrometry; LLOQ, lower limit of quantification.

aMean coefficient of variation of 6.1%.

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