| Gene . | Primer Forward 5´→3´(Tm°) . | Primer Reverse 5´→3´ (Tm°) . | Amplicon Length BP . | Primer Efficiency . | Reference . |
|---|---|---|---|---|---|
| hsp70 | AGTGATCAACGACTCGACACG (65.4°C) | CACTGCATTGGTTATAGTCTTG (58.8°C) | 121 | 93% | Hansen et al., 2006 |
| hsp90aa1 | CGAGGACATGAAGAAGAGGCAT (66.6°C) | ACACTGTCACCTTCTCCACTTT (61.6°C) | 98 | 105% | Meier et al., 2014 |
| cat | CGACGATAACGTCACACAGG (62.3°C) | GCCTGGACCCCGTTTCCATA (70.0°C) | 177 | 101% | Beemelmanns et al., 2021 |
| casp8 | TCCTGTCTATATAAGTGGGCGTTC (64.0°C) | CTTTCCCGAGTGAGCTAACAGT (63.1°C) | 141 | 91% | Beemelmanns et al., 2021 |
| delta-5fad | GTGAATGGGGATCCATAGCA (64.6°C) | AAACGAACGGACAACCAGA (63.29°C) | 192 | 96% | Morais et al., 2011 |
| pfkfb4 | CACGGAAGTGAAAGTGAGCA (64.1°C) | CGTCCAAAGGCACATAGGTT (63.8°C) | 113 | 100% | Caballero-Solares et al., 2018 |
| gapdh | AGGCATCTCACAAACGAGGA (64.8°C) | GGCAACAATCTCAACTCCCT (63.0°C) | 80 | 92% | Meier et al., 2014 |
| Gene . | Primer Forward 5´→3´(Tm°) . | Primer Reverse 5´→3´ (Tm°) . | Amplicon Length BP . | Primer Efficiency . | Reference . |
|---|---|---|---|---|---|
| hsp70 | AGTGATCAACGACTCGACACG (65.4°C) | CACTGCATTGGTTATAGTCTTG (58.8°C) | 121 | 93% | Hansen et al., 2006 |
| hsp90aa1 | CGAGGACATGAAGAAGAGGCAT (66.6°C) | ACACTGTCACCTTCTCCACTTT (61.6°C) | 98 | 105% | Meier et al., 2014 |
| cat | CGACGATAACGTCACACAGG (62.3°C) | GCCTGGACCCCGTTTCCATA (70.0°C) | 177 | 101% | Beemelmanns et al., 2021 |
| casp8 | TCCTGTCTATATAAGTGGGCGTTC (64.0°C) | CTTTCCCGAGTGAGCTAACAGT (63.1°C) | 141 | 91% | Beemelmanns et al., 2021 |
| delta-5fad | GTGAATGGGGATCCATAGCA (64.6°C) | AAACGAACGGACAACCAGA (63.29°C) | 192 | 96% | Morais et al., 2011 |
| pfkfb4 | CACGGAAGTGAAAGTGAGCA (64.1°C) | CGTCCAAAGGCACATAGGTT (63.8°C) | 113 | 100% | Caballero-Solares et al., 2018 |
| gapdh | AGGCATCTCACAAACGAGGA (64.8°C) | GGCAACAATCTCAACTCCCT (63.0°C) | 80 | 92% | Meier et al., 2014 |
Melting temperature Tm°, base pairs BP.
| Gene . | Primer Forward 5´→3´(Tm°) . | Primer Reverse 5´→3´ (Tm°) . | Amplicon Length BP . | Primer Efficiency . | Reference . |
|---|---|---|---|---|---|
| hsp70 | AGTGATCAACGACTCGACACG (65.4°C) | CACTGCATTGGTTATAGTCTTG (58.8°C) | 121 | 93% | Hansen et al., 2006 |
| hsp90aa1 | CGAGGACATGAAGAAGAGGCAT (66.6°C) | ACACTGTCACCTTCTCCACTTT (61.6°C) | 98 | 105% | Meier et al., 2014 |
| cat | CGACGATAACGTCACACAGG (62.3°C) | GCCTGGACCCCGTTTCCATA (70.0°C) | 177 | 101% | Beemelmanns et al., 2021 |
| casp8 | TCCTGTCTATATAAGTGGGCGTTC (64.0°C) | CTTTCCCGAGTGAGCTAACAGT (63.1°C) | 141 | 91% | Beemelmanns et al., 2021 |
| delta-5fad | GTGAATGGGGATCCATAGCA (64.6°C) | AAACGAACGGACAACCAGA (63.29°C) | 192 | 96% | Morais et al., 2011 |
| pfkfb4 | CACGGAAGTGAAAGTGAGCA (64.1°C) | CGTCCAAAGGCACATAGGTT (63.8°C) | 113 | 100% | Caballero-Solares et al., 2018 |
| gapdh | AGGCATCTCACAAACGAGGA (64.8°C) | GGCAACAATCTCAACTCCCT (63.0°C) | 80 | 92% | Meier et al., 2014 |
| Gene . | Primer Forward 5´→3´(Tm°) . | Primer Reverse 5´→3´ (Tm°) . | Amplicon Length BP . | Primer Efficiency . | Reference . |
|---|---|---|---|---|---|
| hsp70 | AGTGATCAACGACTCGACACG (65.4°C) | CACTGCATTGGTTATAGTCTTG (58.8°C) | 121 | 93% | Hansen et al., 2006 |
| hsp90aa1 | CGAGGACATGAAGAAGAGGCAT (66.6°C) | ACACTGTCACCTTCTCCACTTT (61.6°C) | 98 | 105% | Meier et al., 2014 |
| cat | CGACGATAACGTCACACAGG (62.3°C) | GCCTGGACCCCGTTTCCATA (70.0°C) | 177 | 101% | Beemelmanns et al., 2021 |
| casp8 | TCCTGTCTATATAAGTGGGCGTTC (64.0°C) | CTTTCCCGAGTGAGCTAACAGT (63.1°C) | 141 | 91% | Beemelmanns et al., 2021 |
| delta-5fad | GTGAATGGGGATCCATAGCA (64.6°C) | AAACGAACGGACAACCAGA (63.29°C) | 192 | 96% | Morais et al., 2011 |
| pfkfb4 | CACGGAAGTGAAAGTGAGCA (64.1°C) | CGTCCAAAGGCACATAGGTT (63.8°C) | 113 | 100% | Caballero-Solares et al., 2018 |
| gapdh | AGGCATCTCACAAACGAGGA (64.8°C) | GGCAACAATCTCAACTCCCT (63.0°C) | 80 | 92% | Meier et al., 2014 |
Melting temperature Tm°, base pairs BP.
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