Summary of G4 motif occurrences, structure, sequence, and polymerase inhibition
| Variable . | Parallela . | Hybrida . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | GGGT . | L1 . | OGRE . | GGGGCC . | SVA . | GGGA . | FER1L4 . | RRP . |
| Pol ϵ PV (mean) | 5.0 | 2.45 | 7.25*** | 4.26 | 1.35*** | 0.65* | 0.90** | 0.38 |
| Pol δ PV (mean) | 5.2 | 2.06 | 2.19 | 0.76 | 0.99 | 0.43 | 0.56 | 0.41 |
| G tract number | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| G tract lengths (bases) | 3 | 4–6 | 3–5 | 4 | 2–7 | 3 | 3–4 | 3–4 |
| Loop sizes (bases) | 1 | 1 | 1–7 | 2 | 1 | 1 | 3–7 | 2–6 |
| Loop sequences | Y,Y,Y | Y,R,R | mixed | Y,Y,Y | R,R,Y | R | mixed | mixed |
| Occurrences in CHM13v.2 | 871 | 7057 | 0 | 66 | 5323 | 9164 | 1 | 3 |
| Variable . | Parallela . | Hybrida . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | GGGT . | L1 . | OGRE . | GGGGCC . | SVA . | GGGA . | FER1L4 . | RRP . |
| Pol ϵ PV (mean) | 5.0 | 2.45 | 7.25*** | 4.26 | 1.35*** | 0.65* | 0.90** | 0.38 |
| Pol δ PV (mean) | 5.2 | 2.06 | 2.19 | 0.76 | 0.99 | 0.43 | 0.56 | 0.41 |
| G tract number | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| G tract lengths (bases) | 3 | 4–6 | 3–5 | 4 | 2–7 | 3 | 3–4 | 3–4 |
| Loop sizes (bases) | 1 | 1 | 1–7 | 2 | 1 | 1 | 3–7 | 2–6 |
| Loop sequences | Y,Y,Y | Y,R,R | mixed | Y,Y,Y | R,R,Y | R | mixed | mixed |
| Occurrences in CHM13v.2 | 871 | 7057 | 0 | 66 | 5323 | 9164 | 1 | 3 |
aStructures formed in polymerase reaction buffer after thermal denaturation followed by slow annealing (see Fig. 3 and Supplementary Fig. S2).
See Table 1 for G4 motif and flanking sequences. Bold indicates statistically significant differences between Polϵ and Polδ mean PVs for 3–12 independent determinations for each polymerase and motif combination: *P< 0.05; **P< 0.01; ***P< 0.001; unpaired t-test for data sets with equal standard deviations or unpaired t-test with Welch’s correction for data sets with significantly different standard deviations. This comparison for GGGGCC is not significant (P= 0.09), due to the small sample size and high variability of Polϵ PVs for this motif.
Summary of G4 motif occurrences, structure, sequence, and polymerase inhibition
| Variable . | Parallela . | Hybrida . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | GGGT . | L1 . | OGRE . | GGGGCC . | SVA . | GGGA . | FER1L4 . | RRP . |
| Pol ϵ PV (mean) | 5.0 | 2.45 | 7.25*** | 4.26 | 1.35*** | 0.65* | 0.90** | 0.38 |
| Pol δ PV (mean) | 5.2 | 2.06 | 2.19 | 0.76 | 0.99 | 0.43 | 0.56 | 0.41 |
| G tract number | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| G tract lengths (bases) | 3 | 4–6 | 3–5 | 4 | 2–7 | 3 | 3–4 | 3–4 |
| Loop sizes (bases) | 1 | 1 | 1–7 | 2 | 1 | 1 | 3–7 | 2–6 |
| Loop sequences | Y,Y,Y | Y,R,R | mixed | Y,Y,Y | R,R,Y | R | mixed | mixed |
| Occurrences in CHM13v.2 | 871 | 7057 | 0 | 66 | 5323 | 9164 | 1 | 3 |
| Variable . | Parallela . | Hybrida . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | GGGT . | L1 . | OGRE . | GGGGCC . | SVA . | GGGA . | FER1L4 . | RRP . |
| Pol ϵ PV (mean) | 5.0 | 2.45 | 7.25*** | 4.26 | 1.35*** | 0.65* | 0.90** | 0.38 |
| Pol δ PV (mean) | 5.2 | 2.06 | 2.19 | 0.76 | 0.99 | 0.43 | 0.56 | 0.41 |
| G tract number | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| G tract lengths (bases) | 3 | 4–6 | 3–5 | 4 | 2–7 | 3 | 3–4 | 3–4 |
| Loop sizes (bases) | 1 | 1 | 1–7 | 2 | 1 | 1 | 3–7 | 2–6 |
| Loop sequences | Y,Y,Y | Y,R,R | mixed | Y,Y,Y | R,R,Y | R | mixed | mixed |
| Occurrences in CHM13v.2 | 871 | 7057 | 0 | 66 | 5323 | 9164 | 1 | 3 |
aStructures formed in polymerase reaction buffer after thermal denaturation followed by slow annealing (see Fig. 3 and Supplementary Fig. S2).
See Table 1 for G4 motif and flanking sequences. Bold indicates statistically significant differences between Polϵ and Polδ mean PVs for 3–12 independent determinations for each polymerase and motif combination: *P< 0.05; **P< 0.01; ***P< 0.001; unpaired t-test for data sets with equal standard deviations or unpaired t-test with Welch’s correction for data sets with significantly different standard deviations. This comparison for GGGGCC is not significant (P= 0.09), due to the small sample size and high variability of Polϵ PVs for this motif.
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