Table 1. Open in new tab Antibiotic...

Patient		MIC (mg/L)	ST	WGS resistome
1	≥128	16	32	≥32	≥32	16	16	≥16	>32	8	16	≥64	≥16	2	2	0.25	235	VIM-2, aac(6’)-Ib3, aac(6’)-Il, aac(3)-I, aadA6	mexR (nt424Δ11), mexB (S1041G, V1042A), mexZ (E149K), gyrA (T83I)
2	≥128	16	32	16	≥32	4	4	≥16	>32	16	8	4	≥16	≥4	2	0.12	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
3	≥128	16	32	16	≥32	8	8	≥16	>32	8	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
4	≥128	16	16	16	≥32	4	4	≥16	>32	4	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
5	≥128	16	32	16	≥32	8	8	≥16	>32	8	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
6	≥128	≥64	>256	≥32	≥32	8	4	≥16	>32	≥16	128	4	≥16	1	2	0.5	309	VIM-1, CARB-4, aac(6’)-Ib3, aadA1, qnrVC1	mexA (K76Q), mexZ (A61P), mexE (D533E), oprD (nt606insIS256-like), pbpC-PBP3a (T335S), gyrA (T83I)
7	≥128	16	16	16	≥32	4	8	≥16	>32	16	8	4	≥16	≥4	1	0.5	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
8	≥128	16	16	16	≥32	4	4	≥16	>32	8	8	4	≥16	≥4	2	0.12	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

Patient

MIC (mg/L)

WGS resistome

TZP

CAZ

CZA

FEP

CLT

ATM

ATA

IMP

IMR

MEM

MVB

AMK

TOB

CIP

CST

FDC

Acquired resistome

Mutational resistome

≥128

≥32

≥16

>32

≥64

≥16

0.25

235

VIM-2, aac(6’)-Ib3, aac(6’)-Il, aac(3)-I, aadA6

mexR (nt424Δ11), mexB (S1041G, V1042A), mexZ (E149K), gyrA (T83I)

≥128

≥32

≥16

>32

≥16

≥4

0.12

253

VIM-2, aac(6’)-Ib-Hangzhou

mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

≥128

≥32

≥16

>32

≥16

≥4

≤0.5

0.25

253

VIM-2, aac(6’)-Ib-Hangzhou

mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

≥128

≥32

≥16

>32

≥16

≥4

≤0.5

0.25

253

VIM-2, aac(6’)-Ib-Hangzhou

mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

≥128

≥32

≥16

>32

≥16

≥4

≤0.5

0.25

253

VIM-2, aac(6’)-Ib-Hangzhou

mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

≥128

≥64

>256

≥32

≥16

>32

≥16

128

≥16

0.5

309

VIM-1, CARB-4, aac(6’)-Ib3, aadA1, qnrVC1

mexA (K76Q), mexZ (A61P), mexE (D533E), oprD (nt606insIS256-like), pbpC-PBP3a (T335S), gyrA (T83I)

≥128

≥32

≥16

>32

≥16

≥4

0.5

253

VIM-2, aac(6’)-Ib-Hangzhou

mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

≥128

≥32

≥16

>32

≥16

≥4

0.12

253

VIM-2, aac(6’)-Ib-Hangzhou

mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

Patient		MIC (mg/L)	ST	WGS resistome
1	≥128	16	32	≥32	≥32	16	16	≥16	>32	8	16	≥64	≥16	2	2	0.25	235	VIM-2, aac(6’)-Ib3, aac(6’)-Il, aac(3)-I, aadA6	mexR (nt424Δ11), mexB (S1041G, V1042A), mexZ (E149K), gyrA (T83I)
2	≥128	16	32	16	≥32	4	4	≥16	>32	16	8	4	≥16	≥4	2	0.12	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
3	≥128	16	32	16	≥32	8	8	≥16	>32	8	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
4	≥128	16	16	16	≥32	4	4	≥16	>32	4	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
5	≥128	16	32	16	≥32	8	8	≥16	>32	8	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
6	≥128	≥64	>256	≥32	≥32	8	4	≥16	>32	≥16	128	4	≥16	1	2	0.5	309	VIM-1, CARB-4, aac(6’)-Ib3, aadA1, qnrVC1	mexA (K76Q), mexZ (A61P), mexE (D533E), oprD (nt606insIS256-like), pbpC-PBP3a (T335S), gyrA (T83I)
7	≥128	16	16	16	≥32	4	8	≥16	>32	16	8	4	≥16	≥4	1	0.5	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
8	≥128	16	16	16	≥32	4	4	≥16	>32	8	8	4	≥16	≥4	2	0.12	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

Table 1.

Open in new tab

Antibiotic susceptibility profiles, sequence type and WGS resistome of the VIM-producing Pseudomonas aeruginosa isolates

Patient		MIC (mg/L)															ST	WGS resistome
Patient	TZP	CAZ	CZA	FEP	CLT	ATM	ATA	IMP	IMR	MEM	MVB	AMK	TOB	CIP	CST	FDC	ST	Acquired resistome	Mutational resistome
1	≥128	16	32	≥32	≥32	16	16	≥16	>32	8	16	≥64	≥16	2	2	0.25	235	VIM-2, aac(6’)-Ib3, aac(6’)-Il, aac(3)-I, aadA6	mexR (nt424Δ11), mexB (S1041G, V1042A), mexZ (E149K), gyrA (T83I)
2	≥128	16	32	16	≥32	4	4	≥16	>32	16	8	4	≥16	≥4	2	0.12	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
3	≥128	16	32	16	≥32	8	8	≥16	>32	8	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
4	≥128	16	16	16	≥32	4	4	≥16	>32	4	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
5	≥128	16	32	16	≥32	8	8	≥16	>32	8	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
6	≥128	≥64	>256	≥32	≥32	8	4	≥16	>32	≥16	128	4	≥16	1	2	0.5	309	VIM-1, CARB-4, aac(6’)-Ib3, aadA1, qnrVC1	mexA (K76Q), mexZ (A61P), mexE (D533E), oprD (nt606insIS256-like), pbpC-PBP3a (T335S), gyrA (T83I)
7	≥128	16	16	16	≥32	4	8	≥16	>32	16	8	4	≥16	≥4	1	0.5	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
8	≥128	16	16	16	≥32	4	4	≥16	>32	8	8	4	≥16	≥4	2	0.12	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

Patient		MIC (mg/L)															ST	WGS resistome
Patient	TZP	CAZ	CZA	FEP	CLT	ATM	ATA	IMP	IMR	MEM	MVB	AMK	TOB	CIP	CST	FDC	ST	Acquired resistome	Mutational resistome
1	≥128	16	32	≥32	≥32	16	16	≥16	>32	8	16	≥64	≥16	2	2	0.25	235	VIM-2, aac(6’)-Ib3, aac(6’)-Il, aac(3)-I, aadA6	mexR (nt424Δ11), mexB (S1041G, V1042A), mexZ (E149K), gyrA (T83I)
2	≥128	16	32	16	≥32	4	4	≥16	>32	16	8	4	≥16	≥4	2	0.12	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
3	≥128	16	32	16	≥32	8	8	≥16	>32	8	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
4	≥128	16	16	16	≥32	4	4	≥16	>32	4	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
5	≥128	16	32	16	≥32	8	8	≥16	>32	8	8	4	≥16	≥4	≤0.5	0.25	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
6	≥128	≥64	>256	≥32	≥32	8	4	≥16	>32	≥16	128	4	≥16	1	2	0.5	309	VIM-1, CARB-4, aac(6’)-Ib3, aadA1, qnrVC1	mexA (K76Q), mexZ (A61P), mexE (D533E), oprD (nt606insIS256-like), pbpC-PBP3a (T335S), gyrA (T83I)
7	≥128	16	16	16	≥32	4	8	≥16	>32	16	8	4	≥16	≥4	1	0.5	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)
8	≥128	16	16	16	≥32	4	4	≥16	>32	8	8	4	≥16	≥4	2	0.12	253	VIM-2, aac(6’)-Ib-Hangzhou	mexZ (aa102-105Δ), mexD (S915A), pbpA-PBP2 (A481T), gyrA (T83I), parC (S87W)

AMK, amikacin; ATA, aztreonam-avibactam; ATM, aztreonam; CAZ, ceftazidime; CIP, ciprofloxacin; CST, colistin; CZA, ceftazidime-avibactam; CLT, ceftolozane-tazobactam; FEP, cefepime; FDC, cefiderocol; IMP, imipenem; IMR, imipenem-relebactam; MEM, meropenem; MVB, meropenem-vaborbactam; TOB, tobramycin; TZP, piperacillin-tazobactam.

Bold values illustrate susceptible MICs.

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