LQB-118 is effective in vivo, whether locally or systemically delivered, without altering serological markers of toxicity. BALB/c mice were subcutaneously infected with 2 × 10⁶L. amazonensis promastigotes in the rear paws. Animals were treated by intralesional (15 μg/kg/day five times a week) (a) or intraperitoneal or oral delivery (4.5 mg/kg/day, five times a week) (b) of LQB-118. Controls were treated with pentavalent antimonials [sodium stibogluconate in (a) or meglumine antimoniate in (b)], saline or left untreated, as indicated. Lesion development was measured with a dial calliper twice a week. At the end of the experiment the mice were euthanized and the parasite burden was estimated by dilution analysis (inserts). (c) Mice were infected and treated as described in (b). At the end of treatment, serum samples were collected for colorimetric determination of ALT (left panel), AST (middle panel) and creatinine (right panel) concentrations, as parameters of toxicity for the liver and the kidneys. As a positive control, animals were given a single dose of 1% carbon tetrachloride (CCl₄) in peanut oil by an intraperitoneal route. Mean ± SD; n = 6. *P < 0.05, **P < 0.01 and ***P < 0.001. sc, subcutaneous/intralesional; ip, intraperitoneal; NS, not significant among groups.