HIF-1α regulated by Siat7A mediated TAK1 in the cardiomyocytes stimulated by ANG II. (A) Representative immunoblots of expressions of HIF-1α. (B) The mRNA level of HIF-1α in the Siat7A-deleted AC16 cells. (C) Representative immunoblots of HIF-1α expression in the Siat7A-deleted AC16 cells. HIF-1α was down-regulated in AC16 cells. The mRNA level of HIF-1α (D), or TAK1 (E), or BNP (F), or β-MHC (G). (H and I) Representative immunoblots of expressions of HIF-1α, TAK1, ANP, and α-skeletal actin. The results are shown as the mean ± SEM of three independent experiments.