A roadmap of the past of human embryo biopsy: from its dawn, passing through the studies that perfected the protocols, down to the failure of ‘PGS 1.0’. Failure of ‘PGS 1.0’ refers to the evidence of its inefficacy when conducted by fluorescent in situ hybridization (FISH) on a single blastomere. TE, trophectoderm; PB, polar body; WGA, whole genome amplification; PGT, preimplantation genetic testing; PGT-A, PGT for aneuploidies; ZP, zona pellucida; HR, Human Reproduction; Human Gen, Human Genetics; FS, Fertility and Sterility; PNAS, Proceedings of the National Academy of Sciences; JARG, Journal of Assisted Reproduction and Genetics; Prenat Diagn, Prenatal Diagnosis; HRU, Human Reproduction Update.