(A) Absorption spectra, changes in absorbance value of HbA at 630 nm, downward arrow suggests decrease in absorption intensities at both 540 and 580 nm while upward arrow shows increase in absorbance values at 630 nm with increasing concentration of SeNP (successive addition of 10, 20, 35, 50, 70, 90, 120, 150, 200 μl of SeNP). (B) Variation in fluorescence emission intensity of HbA tryptophan at 332 nm while excited at 295 nm, black upward arrow shows increase in fluorescence with intensities increasing concentration of SeNP (successive addition of 5, 15, 35, 50, 100, 200 μl of SeNP). (C) CD spectra of HbA (a), changes in ellipticity at both 208 and 222 nm in presence of 100 μl SeNP (b).