Expression of GLP by qPCR (A–D) and RNA gel-blot (left inset) analysis in three N. attenuata asGLP T2 line (A860-19, A871-16, and A911-10) plants and one wild-type, untransformed line (WT) plant elicited by the following treatments. A, Leaves at nodes 0 undergoing the source-sink transition (L0) and +2 (L + 2), and roots (Roots) of untreated wild-type plants. B, Leaf node +2 of wild-type plants, 24 h after leaves at nodes +2 and +3 were treated with 20 μL of pure lanolin (LC) or 20 μL of lanolin containing 150 μg of MeJA (MJ), MeSA (MS), or IAA; wounded (WD); wounded and treated with 40 μL of 20 mm sodium phosphate buffer (pH 6.5; Buf), 12.5 mm Glc in the buffer (Glc), 25 units mL⁻¹ GOX in the buffer (Gox), or GG, which generates H₂O₂ in situ; or left untreated (C). C, Roots of untreated plants of the three asGLP T2 lines and wild-type plants. D, Leaves growing at node +2 of three N. attenuata asGLP T2 lines and wild-type plants, 4.5 h after leaves at node +2 were wounded four times at 30-min intervals or left untreated (C). Puncture wounds were immediately treated with 6 μL of M. sexta OS (OS). Left inset, The northern-blot results for GLP expressions in leaf node +2 (L + 2) and roots (Roots) of untreated wild-type plants. Right inset, diagram of rosette-stage plant. Asterisks indicate significant differences between members of a pair (each treatment versus its corresponding control; asGLP line versus wild type; P < 0.05, Student's t test).