Physiological performance of the transgenic lines LOHi and LN compared with the wild type (WT) under control and drought stress conditions. A, Relative leaf water content (RWC) estimated at 4 DAS. B and C, Assimilation rate and calculated instantaneous WUE (assimilation [A]/transpiration [E]) in the wild type and transgenic plants. Mean values ± se are given with n = 15 for all the parameters. White and filled bars represent control and stress conditions, respectively. Statistical significant differences among genotypes and a given treatment have been calculated using one-way ANOVA at α = 0.01 with Tukey’s posthoc test. Letters a, b, and c and x, y, and z represent statistical differences under control and stress conditions, respectively. Additional information of two-way ANOVA is provided in Supplemental Table S4.