TLC-immunostaining with anti-Lc₄Cer antibodies of the products of the reaction with Lc₃Cer β-galactosyltransferase. The enzyme reaction was carried out with 10 nmol of Lc₃Cer, 5 μg of Triton CF54, 0.2 M KCl, 20 μM MnCl₂, 1.56 mM UDP-Gal, 50 mM cacodylate-HCl buffer (pH 6.8), and 100 μg of microsomes at 37°C for 2 h, and the products were developed on a TLC plate with chloroform/methanol/0.5% CaCl₂ (55:45:10, by vol.). The heat-denatured microsomal preparation was used as a control. 1, KFr13; 2, KFr13TX; 3, KF28; 4, KF28TX.