Pentamidine treatment prevents mature oligodendrocytes loss and induces recruitment of oligodendrocyte precursor cells at chronic EAE stage. (A) Representative images of the three delineated regions: P, PP and NAWM immunostained for mature oligodendrocytes (MBP) and oligodendrocyte progenitor cells (NG2). Scale bar: 50 µm. Magnification: 40×. Graph bars represent the percentage of area stained for (B) MBP and (C) NG2. The analysis was performed in all experimental groups at 30 days post-EAE induction. Two-way ANOVA with Tukey’s multiple comparisons was used for statistical significance (^#P < 0.05, and ^##P < 0.01 versus EAE) with n = 5 animals per group.