Iron supplementation after MI attenuates LV remodelling. (A) Experimental set-up. Male mice were randomized at 12 weeks after MI to receive either intravenous iron (FCM, 15 µg/g body weight) or saline injections at the indicated time points. Cardiac function was assessed by echocardiography at 12 and 24 weeks and by pressure–volume loops at 24 weeks. (B) Non-haem iron concentration in non-infarcted LV myocardium 24 weeks after MI or sham surgery; n = 10 mice per group. (C) Change in fractional area change (FAC) from 12 to 24 weeks after MI as analysed by echocardiography; n = 10 mice per group. (D) Representative pressure–volume loops at 24 weeks. (E) LV ejection fraction, (F) LV end-diastolic volume, and (G) LV stroke work, as measured by pressure–volume loop catheterization; n = 10 mice per group. (H) OXPHOS complex I (C I) protein expression (normalized to GAPDH); n = 6–7 mice per group, and (I) activity in non-infarcted LV myocardium at 24 weeks; n = 8–9 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001; Mann–Whitney U tests. ^#P < 0.05; Wilcoxon matched paired tests.