Transcriptome analysis of TRV and TRV-RcNAC091 plants. A) PCA of RNA-seq data (PC1, principal component 1; PC2, principal component 2) of TRV and TRV-RcNAC091, respectively. B) Up- and downregulated genes between TRV (TRV-empty vector) and TRV-RcNAC091 plants. The blue bar chart shows the number of significantly upregulated and downregulated genes. C) KEGG analysis of downregulated DEGs between TRV and TRV-RcNAC091 plants. D) Expression analysis of stress-related genes in TRV and TRV-RcNAC091 plants. Eighteen genes were classified into 3 groups, including TF, functional protein, and ABA-responsive genes. RcUBI2 was used as an internal control. Data are the means ± Se of 3 independent experiments. Different letters above the bars indicate significantly different values between TRV and TRV-RcNAC091 for individual genes (P < 0.05), calculated using independent-samples t-test.