Stimulation of ANF638LUX and ANF3003LUX expression by Gα₁₃Q226L. Neonatal cardiac myocytes were transfected with ANF638LUX or ANF3003LUX (15 μg/dish), pON249 (4 μg/dish) and 3 μg of Gα₁₃, Gα₁₃Q226L or pRC/RSV backbone vector (CON) as described under Section 2. After a further 48 h the cells were extracted and assayed for LUX and β-gal expression. The results are the means±S.E.M. from five separate myocyte preparations and are expressed as LUX/β-gal activity ratios relative to transfections with the control vector. Statistical significance by paired t-test: ^aP<0.005, ^cP<0.01 versus control; ^bP<0.005, ^dP<0.02 versus Gα₁₃.