Expression of IL1RAP family receptors and cytokines in human atherosclerotic plaques. Single-cell RNA sequencing data of human carotid plaques (n = 18) from patients that underwent carotid endarterectomy. (A) tSNE depicting 14 distinct cell clusters consisting of T cells, myeloid cells, endothelial cells, smooth muscle cells, B cells, and mast cells (image from Depuydt et al.¹⁵). (B) Feature plot of IL1RAP gene expression. Dot plots of gene expression of (C) IL1RAP-related receptors and (D) IL1RAP-related cytokines. (E) Human carotid plaques (n = 4) were digested, stained with anti-IL1RAP-Alexa Fluor 647 or isotype control IgG, and analysed for IL1RAP MFI by flow cytometry. ΔMFI defined as the difference in MFI in each cell population between anti-IL1RAP and isotype antibody staining. (F) Representative histogram of IL1RAP expression on carotid plaque CD14⁺ myeloid cells.