Recombinant human extracellular histones accelerate Ca/Pi-dependent calcification in MOVAS cells. (A) The representative image of Alizarin red S-stained MOVAS cells treated with calcification medium with extracellular histones (20 μg/ml; Day 7). n = 3 per group. (B) Plotted to mean the dye of Alizarin red S. n = 5 per group. All data are presented as the mean ± SEM. ***P < 0.001. n.s., not significant. One-way ANOVA followed by Tukey’s post hoc test. CaP: calcium and phosphate (Ca/Pi).