Comparison of the primary structures of CGL1 and CG13321. (A) CGL1 and CG13321 consist of two and four DM9 motifs, respectively, labeled as D1 to D4. (B) Alignment of the DM9 motifs in CGL1 and CG13321. The amino acid sequences of each DM9 motif of both proteins were aligned using Clustal W (26). Asterisks, colons and periods indicate identical, strongly similar and weakly similar residues, respectively. The residues in CGL1 that form hydrogen bonds with the hydroxy groups of mannose, along with their corresponding residues in CG13321, are enclosed in red boxes. The residues involved in CH/π interactions in CGL1 and their corresponding residues in CG13321 are enclosed in blue boxes.