Affinity chromatography of rCG13321 using the mannose-cellufine column. (A) SDS-PAGE of expressed rCG13321 in E. coli. rCG13321 was expressed in both the supernatant and precipitate after centrifugation of the lysate of the cells. The soluble proteins in the supernatant were subjected to affinity chromatography on the mannose-cellufine. (B) Elution profile of the mannose-Cellufine column chromatography. SDS-PAGE indicated that purified proteins of 31 kDa were eluted in the tailing and adsorbed fractions, suggesting that there were two forms of rCG13321 (LA and HA) differing in affinity for the column.