Violin plots of CXCL8 expression in TPM for CTL and FTR groups divided by anatomical location of leaflet (A). Expression of CXCL8 (TPM) in FTR animals with mild, moderate or severe TR (B). Scatter plot of CXCL8 (TPM) indicating correlations between gene expression and TAPSE with regression trend lines for anterior (orange), posterior (blue) and septal (green) leaflets. CTL: control; CXCL8: IL-8; DEG: differentially expressed genes; FTR: functional tricuspid regurgitation; TAPSE: tricuspid annular plane systolic excursion; TPM: transcripts per million; TR: tricuspid regurgitation. *P < 0.05).