Nuclear and mitochondrial phylogeographic trees. Midrooted distance tree derived from filtered nuclear genotype likelihoods (a) and a maximum likelihood tree from aligned reconstructed mitochondrial assemblies (b), with colors representing the two species and shapes corresponding to the source location (Northern DPS, Southern DPS, allopatric, and sympatric). For both trees, colors in the diagrammed silhouettes correspond to the LEPC (T. pallidicinctus) and the GRPC (T. cupidio). The red arrows in a) identify the division between LEPC collected from the Northern and Southern DPSs, and the labeled sample numbers represent the potentially misidentified or introgressed birds illustrated in the bar plot above Figure 1b. The goldenrod arrows in b) are illustrated to orient the location of several GRPC samples throughout the mtDNA tree. The nuclear tree (a) shows increased species discrimination compared to the mitochondrial tree (b), but neither exhibits a clear pattern of reciprocal monophyly that is often associated with distinct species or evolutionarily significant units (22) driven in part by bidirectional introgression between species.
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