Experimental validation of hub genes and the potential role of ENTPD3. (A) Gene expressions (log2 transformed) of ENTPD3, ENPP3, PTGS2, and SST in RIFs and controls based on microarray data. (B) The mRNA expressions of ENTPD3, ENPP3, PTGS2, and SST in RIFs and controls were measured by qRT-PCR. (C) The mRNA expressions of ENTPD3, CDH1, CDH2, and VIM in ENTPD3 knockdown samples and controls. (D) Protein levels of ENTPD3, E-cadherin, N-cadherin, and vimentin in ENTPD3 knockdown samples and controls. (E) Immunofluorescence staining against TNF-α and IL-1β in endometria from RIFs and controls. Scale bar, 100 μm. (F) The mRNA expressions of TNF and IL1B in ENTPD3 knockdown samples and controls. Statistical significance was determined by a two-sided unpaired Student’s t-test: ***P < 0.001; **P < 0.01; *P < 0.05; ns, not significant.
