Analysis summary tab. The top frame shows the total numbers of differentially expressed genes and differentially spliced isoforms under different experimental conditions as well as the numbers of associated GO terms for which these genes are overrepresented. We define genes with at least one isoform that was assigned a high probability of proportion changes between experimental conditions as differentially expressed. In addition, the number of observed genes is shown, which corresponds to the number of genes that had at least two expressed isoforms. Finally, the FDR shown corresponds to the mean of the PEP of the smallest set of differentially expressed or differentially spliced genes that satisfies the cut-off (0.05). The bottom frame provides statistics for each gene in the dataset, and the user can visualize the structure of a gene by clicking the ‘Visualize’ button (see Figure 3). The column ‘Fold Change’ provides the log2 fold change of the gene expression. The column ‘PEP (Splicing)’ provides the PEP of differential splicing.
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