The transcript levels of orthologous genes classified as ManS and GMGT genes. (A) RNA-seq of ManS orthologs. (B) RNA-seq of GMGT orthologs. Gene expression levels are shown as normalized TPM values. (C) RT-qPCR of transcript levels of the predicted ManS gene (g23576.t1) and the predicted GMGT gene (g31248.t1). Total RNA was extracted from stems, leaves, calli, and endosperm and subjected to RT-qPCR. The transcript levels were normalized to PP4L transcript levels. Error bars represent SD of 3 biological repeats (*P < 0.01).