Architecture of Hera and constructs used in this study. (A) Domain architecture of Hera. The helicase core comprising the N-terminal and C-terminal RecA domains (RecA_N, RecA_C; orange, blue) carries the conserved motifs [Q, I, Ia, Ib, Ic, II (DEAD-box), and III in RecA_N, and IV, IVa, V, Va, and VI in RecA_C]. The C-terminal extension is formed by the DD (black) and the RBD (red). (B) Structural model of the Hera dimer [17, 19, 20]. The RecA_N and RecA_C of the helicase core are shown in orange and blue, the DD in black, and the RBD in red. The cartoon depiction used in the following is shown on the right. (C) Constructs used in this study and color code used throughout the manuscript for data sets obtained for these constructs. The color code for the domains is the same as in panels (A) and (B).