Glycopenia-induced counter-regulatory mechanisms are regulated by pancreatic but not neuronal K_ATP channels. (A-C) Blood glucose levels during 2DG-induced glycopenia assessment in WT vs Kir6.2 KO (A), Nestin^Cre/0; Kir6.2^flox/+ vs ^flox/flox (B), and Ins1^Cre/0; Kir6.2^flox/+ vs ^flox/flox mice (C). (D-E) Plasma glucagon (D) and insulin concentration (E) after a 6-hour fasting duration (Before 2DG injection) and 30 minutes after 2DG injection (After 2DG injection) in WT, Kir6.2 KO, Ins1^Cre/0; Kir6.2^flox/+, and Ins1^Cre/0; Kir6.2^flox/flox mice. WT: n = 8 (A), 10 (D, E); Kir6.2 KO: n = 8 (A), 9 (D), 10 (E); Ins1^Cre/0; Kir6.2^flox/+: 5 (C, E), 4 (D); Ins1^Cre/0; Kir6.2^flox/flox: n = 10 (C), 5 (D), 6 (E); Nestin^Cre/0; Kir6.2^flox/+: n = 10 (B); Nestin^Cre/0; Kir6.2^flox/flox: n = 8 (B). Statistical comparisons were made by two-way ANOVA with Šídák’s correction (A-C) and two-way ANOVA with Tukey’s correction followed by multiple comparisons (D-E). Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: not significant.