Adcy3 null mice exhibit impaired elevation of circulating catecholamines by chronic intermittent hypoxia (CIH). (A) Representative high-pressure liquid chromatography (HPLC) elution profiles of plasma norepinephrine (NE) and epinephrine (EPI) from Adcy3^+/+ and Adcy3^−/− mice subjected to either room air (NOR) or 10 days of CIH. The elution times for NE and EPI were 6.9 and 8.1 min, respectively. (B and C) Average (mean ± SEM) and individual data of NE (B) and Epi (C) from both genotypes subjected to either room air (CON) or CIH. n = 7 mice in each group. ***P < .001; *P < .05; n.s. not significant P > .05 was analyzed with 2-way ANOVA followed by the Holm-Sidak test. (B) Main factor effect of genotype, P = .001; main factor effect of treatment (CON vs. CIH), P < .001; interaction effect (genotype × treatment), P = 0.088. (C) Main factor effect of genotype, P = 0.177; main factor effect of treatment (CON vs. CIH), P = .058; interaction effect (genotype × treatment), P = .039.