Negative conflict behavior moderates visit-related differences in blood pressure. Negative behavior during the marital conflict interacted with visit to predict systolic blood pressure (SBP) (P = .037). At both high and low levels of negative behavior, spouses’ pre-conflict SBP was higher at Visit 1 than Visit 2, suggesting anticipatory habituation, but there were no visit differences in post-conflict SBP. As a result, spouses’ SBP significantly decreased from pre- to post-conflict at Visit 1 but not at Visit 2, and this decline was steeper for couples with low levels of hostile behavior.