DDIT3 is essential for the development of bone marrow B cells and differentiation of peripheral B cells. (A–C) Representative flow diagrams and statistical analysis of pre-pro (a), pro (b), early-pre (c), late-pre (d), immature (e), and recirculating mature (f) B-cell in bone marrow from WT (n = 6) and DDIT3 KO mice (n = 6). (D) Statistical analysis of MFI of CD127 in pre-pro (a), pro (b), early-pre (c), late-pre (d), immature (e), and recirculating mature (f) B-cell in bone marrow from WT (n = 6) and DDIT3 KO mice (n = 6). (E–H) Representative flow diagrams and statistical analysis of splenic GC and MZ B cells in WT (n = 6) and DDIT3 KO (n = 6) mice. (I–L) Representative flow diagrams and statistical analysis of splenic GC and MZ B cells in CD45.2⁺ and CD45.1⁺ cells from chimera mice (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001, unpaired student’s t-test.