DDIT3 deficiency ameliorates lupus-like autoimmunity and renal injury in a cGVHD model. (A) ELISA analysis for anti-dsDNA antibody in serum from BM12 induced-WT (n = 7) and KO mice (n = 6) for 4 weeks. (B) Serum BUN in WT (n = 7) and KO (n = 6) mice. (C) BCA analysis of urinary protein in WT (n = 7) and KO (n = 6) mice. (D) Urinary creatinine in WT (n = 7) and KO (n = 6) mice. (E) Ratio of urinary protein to urinary creatinine in WT (n = 7) and KO (n = 6) mice. (F) Immunofluorescence staining of C3 and total IgG in kidneys from induced WT and KO mice. Scale bar, 10 μm. (G) H&E analysis of kidneys from WT and KO mice. Scale bar, 250 μm. (H) Immunofluorescence staining of GCs from WT and KO mice. Shown are representative GCs using anti-CD4 (blue), anti-B220 (green), and anti-GL-7 (red). Scale bar, 20 μm. (I) Quantification of the area of GCs from WT (n = 3) and KO (n = 3) mice. (J–N) Representative flow diagrams and statistical analysis of GCB, PBC, and PC in BM12 induced WT (n = 5) and KO (n = 5) mice. (O–R) Flow cytometry analysis of CD80, CD86, and CD69 expression in B cell from BM12-induced WT (n = 5) and KO mice (n = 5). ns = no significance, *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001, unpaired student’s t-test.