Discovery and activity analysis of ODD enzymes. (A) Predicted epoxidation function of ODD. (B) ODD gene family tree (showing the distribution and evolutionary relationships of the identified genes). (C) Chromosomal distribution of paclitaxel synthesis-related ODD enzymes. Purple circles indicate all ODDs in TWv1, and green circles indicate candidate genes screened by coexpression analysis. (D) Heatmap of coexpression of genes related to the paclitaxel synthesis pathway. (E) Relative activity assay of the epoxidation function of the 11 candidate genes. (F) Comparison of catalytic distance and activity of the 11 candidate genes. Green indicates catalytic activity; gray indicates no catalytic activity. (G) Multiple sequence alignment analysis of the 11 candidate genes. (H) Analysis of hydrogen bond formation in loop 86–101 of the catalytic pocket of sequence 15. (I) (a) Catalytic pocket of sequence 15, and (b) catalytic pocket of sequence 10.