Colonization and quantification E. coli Nissle (EcN) in the gut of slc26a3^+/+ and slc26a3^−/− mice: A, Schematic diagram of the experimental protocol. EcN primarily colonized the gut of the slc26a3^−/− mice when compared to slc26a3^+/+ mice with a slightly higher amount in (B) cecal content compared to (C) fecal content. Data are shown as EcN counts/g content and as a ratio to total bacteria counts. N = 3-4 per group. Data are represented as mean ± SEM. Statistical differences were calculated using unpaired Student’s t-tests or nonparametric Mann-Whitney U-tests. # represents the statistics performed between the 2 different genotypes. ^#P < .05.