Effect of E. coli Nissle (EcN) on the levels of short-chain fatty acid (SCFA) and its transporters in slc26a3^+/+ and slc26a3^−/− mice: A, A modest but significant increase in the total SCFA, acetate, propionate, and butyrate in the cecal content was observed in the slc26a3^+/+ and slc26a3^−/− mice after gavage with EcN. B, The total SCFA increased only in the slc26a3^+/+ mice along with propionate showing a significant increase in the fecal content in both the groups after gavage with EcN. C and D, mRNA expression of SCFA transporters Mct1 and Smct1 increased significantly in the slc26a3^−/− mice after gavage in the cecum, while it remained unchanged and similar to the slc26a3^+/+ levels in the distal colon. N = 7-17 per group, each dot represents 1 mouse. Data are represented as mean ± SEM. Statistical differences were calculated using unpaired Student’s t-tests or nonparametric Mann-Whitney U-tests. # represents the statistics performed between the 2 different genotypes, and * represents the statistics performed between the same genotype posttreatment. # and * P < .05.