Respirometry experimental schematic. Intermittent-flow respirometry was utilized for determination of routine and maximum oxygen consumption (ṀO₂) in JD (n = 14), RBD (n = 14) and FTD (n = 14) darters, following five different temperature ramps. All fish were placed into chambers at 15°C overnight prior to measurement. Each species set rested for 1 week following trial. Created in BioRender. Craig, P. (2025) https://BioRender.com/nrvyufl