Time course of mtRNAP DragonRNA reactions. Reactions run under “Lu conditions” with 5′ FAM-labeled AF-EG-15 were analyzed via 10% TBE-urea gel electrophoresis. (A) Sequence of oligonucleotide AF-EG-15 used in reactions shown in panels (B) and (C). (B) Twenty-four hour time course of hmtRNAP DragonRNA reactions. (C) Three-hour time course of Rpo41 DragonRNA reactions. FAM-label imaging is shown overlaid with a 50% transparent image of nucleic acid stain of the same reaction. Images were independently contrast-enhanced and overlaid in PowerPoint, separate images are in Supplementary File S1.