TPM4 promotes contractions in primary myometrium cells. (A) Transfection efficiency in primary myometrium cells with specific TPM4 siRNAs and its overexpression plasmid. (B) 3D collagen gel matrix applied after TPM4 siRNA#2 and plasmid transfected. (C) Progesterone receptors (PRs) and contraction-associated proteins (CAPs) mRNA were detected by quantitative real-time PCR (qRT-PCR). (D) PRs and CAPs proteins were analyzed by western blotting. The gray values were quantified with the analysis software. (E) Rescue experiment was done by using GAS5 plasmid and TPM4 siRNA, and the contraction ability was significantly inhibited (Figure 6E). *P < .05, ^#P < .05.