Figure 5.
Comparison of the expression of BAT/beige adipose tissue marker genes in the subcutaneous adipose tissue of WT and M4-KO mice. (A-H) Differential expression patterns of Tbx1, Ebf3, Ear2/Nr2F6, Ucp1, Pdgfa, B3ar/Adrb3R, Sp100, and Car4 in the subcutaneous adipose tissue of M4-KO mice. The results of RNA-Seq are based on 3 independent experiments and are presented as the mean ± SD. Data were analyzed using a 2-tailed Student's t-test. *P < .05. (I) Relative quantification of the expression level of Tbx1, Ebf3, Ear2/Nr2F6, Ucp1, Pdgfa, B3ar/Adrb3R, Sp100, and Car4 in the subcutaneous adipose tissue of M4-KO mice. The results of qRT-PCR are based on 3 independent experiments and are presented as the mean ± SD. Data were analyzed using a 2-tailed Student's t-test. ***P < .001.

Comparison of the expression of BAT/beige adipose tissue marker genes in the subcutaneous adipose tissue of WT and M4-KO mice. (A-H) Differential expression patterns of Tbx1, Ebf3, Ear2/Nr2F6, Ucp1, Pdgfa, B3ar/Adrb3R, Sp100, and Car4 in the subcutaneous adipose tissue of M4-KO mice. The results of RNA-Seq are based on 3 independent experiments and are presented as the mean ± SD. Data were analyzed using a 2-tailed Student's t-test. *P < .05. (I) Relative quantification of the expression level of Tbx1, Ebf3, Ear2/Nr2F6, Ucp1, Pdgfa, B3ar/Adrb3R, Sp100, and Car4 in the subcutaneous adipose tissue of M4-KO mice. The results of qRT-PCR are based on 3 independent experiments and are presented as the mean ± SD. Data were analyzed using a 2-tailed Student's t-test. ***P < .001.

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