Maximum tolerated dose study. (A) C57BL/6 mice were given a single immunization with 5 µg (pink squares), 10 µg (teal triangles), or 15 µg (purple diamonds) of EMT4™ and weighed through day 12 along with untreated cohorts (black circles). (B) In a separate study cohorts were given 4 consecutive immunizations with 5 µg of EMT4™ (purple squares), or 5 µg of a commercial TLR4 agonist (blue hexagons) and weighed through day 12 along with untreated cohorts (black circles). Dosing intervals are denoted by open upside down triangles along the X-axis. Treated cohorts were individually compared to untreated using a 2-way ANOVA with Dunnetts multiple comparisons, significant differences are denoted where *P < 0.05. Data representative of one experiment with n = 4 animals per group.