MCU suppression caused nucleoid enlargement in HT1080 cells. (A) HT1080 cells were treated with indicated siRNA for 96 h and then observed using a DMi8 fluorescence microscope. Representative images were shown. The white dashed square indicates the magnified area. (B) Protein levels in the MCU-knockdown HT1080 cells were determined by immunoblotting using the indicated antibodies. (C, D) The maximum nucleoid size (C) and nucleoid number (D) in each cell of (A) were quantified using ImageJ. All graphs show plots of the raw data and the mean ± SD. n ≥ 3 for each quantitative dataset. All statistical analyses were performed using the Tukey–Kramer test. ^**P < 0.01.