LAMP2A plays a key role in CMA-mediated inflammation of microgila cells during ischemia-induced SCI. A) Immunohistological staining of LAMP2A in the perforntal cortex (upper panel) and hippocampus (lower panel) of rats from control group (ctrl), sham operated group (Sham) and SCI group (SCI), and integrated optical density (IOD) (right panel) was calculated. Representative images were from n = 3. Scale bar, 100 μm. B) Immunoblot of LAMP2A in the total cell lysate or lysosome of LAMP2A in microgila cells isolated from the rat brains of different groups, β-actin and Hsc70 were used as loading control. Representative blots were from n = 3. C) Ratio of LAMP2A/β-actin, LAMP2A/LAMP1 and Hsc70/LAMP1 based on densitometric analysis of immunoblot. n = 3. D) Native-PAGE of microgila cells isolated from sham and SCI rats. GAPDH was detected as loading control. Representative blots were from n = 3. E) The ratio of LAMP2A/GAPDH based on native-PAGE. n = 3. F) Real-time quantitative PCR of iNOS, Arg1 and Ym1/2 in microgila cells isolated from the spinal cord injured site of different groups. n = 3. ^*, P < 0.05 compared to control and sham; ^**P < 0.01 compared to control and sham. For the comparison between two groups, we used student’s t test; for the comparison among three groups, we used 2-way ANOVA following Tukey’s multiple comparisons test.