p38 MAPK is activated in the lysosome of microglia cells during ischemia-induced SCI. A) Immunoblotting of MEF2D, GAPDH, p-p38α, p38α, p-ATF2 and ATF2 in microgila cells isolated from different groups, and then treated with or without SB203580. Representative blots were from n = 3. B) Densitometric quantification of MEF2D according to immunoblotting, and the calculation of the ratio of p-p38α/p-38α and p-AFT2/AFT based on immunoblotting. n = 3. C) Immunoblotting of p-p38α, p38α, PDI and LAMP1 in the lysosome fractions from microglia cells of different groups. Representative blots were from n = 3. D) The ratio of p-p38α/p38α and PDI/LAMP1 according to immunoblotting. n = 3. ^*P < 0.05 compared to control and sham; ^**P < 0.01 compared to control and sham; ^***P < 0.001 compared to sham. 2-way ANOVA following Tukey’s multiple comparisons test.